Mass spectrometry-based glycomic profiling of the total IgG and total proteome N-glycomes isolated from follicular fluid
نویسندگان
چکیده
منابع مشابه
High-throughput quantitative profiling of serum N-glycome by MALDI-TOF mass spectrometry and N-glycomic fingerprint of liver fibrosis.
BACKGROUND The use of MALDI-TOF mass spectrometry (MS) in quantitative glycan profiling has not been reported. In this study, we attempted to establish a high-throughput quantitative assay for profiling serum N-glycome, and we applied the new assay to identifying serum N-glycans for diagnosis of liver fibrosis and cirrhosis. METHODS N-glycans from whole serum proteins in 2 microL serum were r...
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the aim of the present study was to determine the in?uence of presence or absence of corpus luteum (cl) on hormonal and metabolites composition of follicular ?uid (ff) harvested from different sized follicles and its relationship with blood serum concentrations in sanjabi ewes. ovaries and blood samples were collected from 60 clinically healthy adult ewes (sanjabi breed) 1–3 years of age in dio...
Changes in IgG and total plasma protein glycomes in acute systemic inflammation
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متن کاملMass Spectrometry-based Methods of Proteome Analysis
1 Principles and Instrumentation 3 1.1 Proteome and Proteomics 3 1.2 Need for Large-scale Analyses of Gene Products at the Protein Level 3 1.3 General Problems in Proteome Analyses 4 1.4 MS Instrumentation 5 1.4.1 Ionization Methods 6 1.4.2 Mass Analyzers 8 1.4.3 MS/MS 9 1.5 Methods of Sample Fractionation 10 1.5.1 2D-electrophoretic Separation of Complex Protein Mixtures 11 1.5.2 Liquid-phase ...
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ژورنال
عنوان ژورنال: Biomolecular Concepts
سال: 2020
ISSN: 1868-503X,1868-5021
DOI: 10.1515/bmc-2020-0015